A simple medium enables bovine embryos to be held for seven days at 4°C

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A simple medium enables bovine embryos to be held for seven days at 4°C

Cryopreservation methods using liquid nitrogen (LN(2)) for gametes and embryos are prevalent in mammalian artificial reproduction. However, the pregnancy rate from frozen embryos has not improved over the past two decades because freeze-thawing causes significant damage. The strict regulation of transportation of LN(2) containers by airlines also limits exchange between breeders. In this articl...

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Differential Effect of Medium on the Ratio of ICM/TE of Bovine Embryos in a Co-culture System

Background This study was undertaken to investigate the efficiency of two different embryo somatic cell co-culture conditions tissue culture medium 199 (TCM199)–vero cells and Menezo B2 (B2)-vero cells for the in vitro developmental quantity and quality of bovine embryos. MaterialsAndMethods Bovine oocytes were allowed to mature and subsequently undergo fertilization in vitro. Their presumptive...

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ژورنال

عنوان ژورنال: Scientific Reports

سال: 2013

ISSN: 2045-2322

DOI: 10.1038/srep01173